PMID:10339822
| Citation |
Elssner, T, Preusser, A, Wagner, U and Kleber, HP (1999) Metabolism of L(-)-carnitine by Enterobacteriaceae under aerobic conditions. FEMS Microbiol. Lett. 174:295-301 |
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| Abstract |
Different Enterobacteriaceae, such as Escherichia coli, Proteus vulgaris and Proteus mirabilis, are able to convert L(-)-carnitine, via crotonobetaine, into gamma-butyrobetaine in the presence of carbon and nitrogen sources under aerobic conditions. Intermediates of L(-)-carnitine metabolism (crotonobetaine, gamma-butyrobetaine) could be detected by thin-layer chromatography. In parallel, L(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase activities were determined enzymatically. Monoclonal antibodies against purified CaiB and CaiA from E. coli O44K74 were used to screen cell-free extracts of different Enterobacteriaceae (E. coli ATCC 25922, P. vulgaris, P. mirabilis, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae) grown under aerobic conditions in the presence of L(-)-carnitine. |
| Links | |
| Keywords |
Acyltransferases; Aerobiosis; Antibodies, Monoclonal/immunology; Blotting, Western; Carnitine/metabolism; Electrophoresis, Polyacrylamide Gel; Enterobacteriaceae/enzymology; Enterobacteriaceae/growth & development; Hydro-Lyases/immunology; Hydro-Lyases/metabolism; Multienzyme Complexes/immunology; Multienzyme Complexes/metabolism; Oxidoreductases/immunology; Oxidoreductases/metabolism |
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