PMID:10322040
| Citation |
Patzer, SI and Hantke, K (1999) SufS is a NifS-like protein, and SufD is necessary for stability of the [2Fe-2S] FhuF protein in Escherichia coli. J. Bacteriol. 181:3307-9 |
|---|---|
| Abstract |
Escherichia coli fhuF mutants, a sufS::MudI mutant, and a sufD::MudI mutant were found to have the same phenotype: the inability to use ferrioxamine B as an iron source in a plate assay. In addition, the sufS and sufD genes were shown to be regulated by the iron-dependent Fur repressor. Sequence analysis revealed that the sufS open reading frame corresponds to orf f406. The protein SufS belongs to the family of NifS-like proteins, which supply sulfur for [Fe-S] centers. The protein FhuF contains a [2Fe-2S] center. A mutation in the upstream sufD gene (orf f423) caused the same phenotype. The T7 expression system and a His tag allow the isolation in good yield of the FhuF protein from a wild-type strain. In contrast, overproduction of the protein in a DeltasufD strain failed. Radioactive labeling of N-His-FhuF with [35S]methionine showed that the protein was unstable in the DeltasufD mutant. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Bacterial Proteins/physiology; Deferoxamine/metabolism; Escherichia coli/drug effects; Escherichia coli/genetics; Ferric Compounds/metabolism; Gene Expression Regulation, Bacterial/drug effects; Genes, Reporter/genetics; Iron/pharmacology; Iron-Binding Proteins; Iron-Sulfur Proteins/genetics; Iron-Sulfur Proteins/isolation & purification; Iron-Sulfur Proteins/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Open Reading Frames/genetics; Operon/genetics; Periplasmic Binding Proteins; Physical Chromosome Mapping; Promoter Regions, Genetic/genetics; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Repressor Proteins/metabolism; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Temperature |
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