PMID:10322034
| Citation |
Dekker, N, Tommassen, J and Verheij, HM (1999) Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo. J. Bacteriol. 181:3281-3 |
|---|---|
| Abstract |
Bacteriocin release protein is known to activate outer membrane phospholipase A (OMPLA), which results in the release of colicin from Escherichia coli. In vivo chemical cross-linking experiments revealed that the activation coincides with dimerization of OMPLA. Permeabilization of the cell envelope and dimerization were characterized by a lag time of 2 h. |
| Links | |
| Keywords |
Alkaline Phosphatase/metabolism; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/biosynthesis; Bacterial Proteins/physiology; Cell Membrane Permeability; Chloramphenicol O-Acetyltransferase/metabolism; Cross-Linking Reagents; Cytosol/enzymology; Cytosol/metabolism; Dimerization; Enzyme Activation; Escherichia coli/cytology; Escherichia coli/enzymology; Escherichia coli/metabolism; Lipoproteins; Models, Biological; Periplasm/enzymology; Periplasm/metabolism; Phospholipases A/genetics; Phospholipases A/metabolism; Protein Binding; Time Factors |
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