PMID:10231484
| Citation |
Di Lallo, G, Anderluzzi, D, Ghelardini, P and Paolozzi, L (1999) FtsZ dimerization in vivo. Mol. Microbiol. 32:265-74 |
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| Abstract |
A hybrid assay, based on the properties of the lambda repressor, was developed to detect FtsZ dimerization in Escherichia coli in vivo. A gene fusion comprising the N-terminal end of the lambda cI repressor gene and the complete E. coli ftsZ gene was constructed. The fused protein resulted in a functional lambda repressor and was able to complement the thermosensitive mutant ftsZ84. Using the same strategy, a series of 10 novel mutants of FtsZ that are unable to dimerize was selected, and a deletion analysis of the protein was carried out. Characterization of these mutants allowed the identification of three separate FtsZ portions: the N-terminal of about 150 amino acids; the C-terminal of about 60 amino acids, which corresponds to the less conserved portion of the protein; and a central region of about 150 residues. Mutants belonging to this region would define the dimerization domain of FtsZ. |
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| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage lambda/physiology; Blotting, Western; Cytoskeletal Proteins; Dimerization; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli/virology; Gene Deletion; Mutation; Plasmids/genetics; Polymerase Chain Reaction/methods; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Recombination, Genetic |
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