PMID:10229930

Citation	Ogawa, HI, Ohyama, Y, Ohsumi, Y, Kakimoto, K, Kato, Y, Shirai, Y, Nunoshiba, T and Yamamoto, K (1999) Cobaltous chloride-induced mutagenesis in the supF tRNA gene of Escherichia coli. Mutagenesis 14:249-53
Abstract	The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent nalidixic acid-resistant, ampicillin-resistant and Lac+ (SupF-) clones were obtained and the altered sequences of the mutated supF genes were determined. Deletions and frameshifts were the predominant mutational event (61%) induced by CoCl2 and base substitutions were induced to a lesser degree (29%). Analysis of sequence alterations at all the sites of mutation revealed that: (i) 18 of 19 base substitutions and eight of 10 frameshifts occurred at G:C sites, suggesting that the formation of N7G-Co(II) adducts may be responsible for premutagenic lesions of these mutations; (ii) short sequence repeats were mostly found at the sites of deletions and frameshifts. Slippage-misalignment is also suggested to be a mechanism for the induction of mutations at these sites.
Links	PubMed
Keywords	Antimutagenic Agents/toxicity; Cobalt/toxicity; DNA Transposable Elements; Escherichia coli/genetics; Frameshift Mutation; Gene Deletion; Genes, Suppressor; Models, Genetic; Mutagenesis; Point Mutation; RNA, Transfer/drug effects; Sequence Analysis, DNA
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