PMID:10227169

Citation	Genevaux, P, Bauda, P, DuBow, MS and Oudega, B (1999) Identification of Tn10 insertions in the dsbA gene affecting Escherichia coli biofilm formation. FEMS Microbiol. Lett. 173:403-9
Abstract	Escherichia coli was used as model to study initial adhesion and early biofilm development to an abiotic surface. Tn10 insertion mutants with reduced attachment to a polystyrene surface were isolated. Three adhesion mutants harbored the transposon in the dsbA gene, whose product, DsbA, catalyses folding of numerous extracytoplasmic disulfide bond-containing proteins. All three mutants were weakly adherent and grew poorly. Cell surface structure analysis showed that motility. type 1 fimbriation and lipopolysaccharide structure were affected in these mutants. The pleiotropic effect of the dsbA mutations on biofilm formation is discussed.
Links	PubMed
Keywords	Bacterial Adhesion; Biofilms/growth & development; DNA Transposable Elements; Escherichia coli/genetics; Escherichia coli/physiology; Fimbriae, Bacterial/chemistry; Fimbriae, Bacterial/metabolism; Genes, Bacterial; Lipopolysaccharides/chemistry; Lipopolysaccharides/metabolism; Mutation; Periplasm; Polymerase Chain Reaction/methods; Polystyrenes; Protein Disulfide-Isomerases/genetics; Sequence Analysis, DNA; Surface Properties
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