PMID:10227163

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Citation

Macdonald, AG and Martinac, B (1999) Effect of high hydrostatic pressure on the porin OmpC from Escherichia coli. FEMS Microbiol. Lett. 173:327-34

Abstract

Porin OmpC from Escherichia coli was reconstituted in liposomes and its gating kinetics were recorded at high hydrostatic pressure, up to 90 MPa, using a development of the patch clamp technique. The composition of the recording solution influenced the results but generally high hydrostatic pressure favoured channel opening.

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Keywords

Escherichia coli/chemistry; Hydrostatic Pressure; Ion Channel Gating; Kinetics; Liposomes; Patch-Clamp Techniques; Porins/isolation & purification; Porins/physiology

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