PMID:10216301
| Citation |
Newberry, KJ, Kohn, J, Hou, YM and Perona, JJ (1999) Crystallization and preliminary diffraction analysis of Escherichia coli cysteinyl-tRNA synthetase. Acta Crystallogr. D Biol. Crystallogr. 55:1046-7 |
|---|---|
| Abstract |
Crystals of the 52 kDa monomeric Escherichia coli cysteinyl-tRNA synthetase complexed with ATP and cysteine have been grown by hanging-drop vapor diffusion from solutions containing ammonium sulfate as the precipitating agent. The crystals form long hexagonal rods in the space group P321 with unit-cell dimensions a = b = 82.3, c = 168.9 A. There is one enzyme molecule in the asymmetric unit. A complete native data set has been collected from a rotating-anode source to a resolution of 2.7 A at 103 K, with an Rmerge of 6.7%. |
| Links | |
| Keywords |
Amino Acyl-tRNA Synthetases/chemistry; Amino Acyl-tRNA Synthetases/isolation & purification; Bacterial Proteins/chemistry; Bacterial Proteins/isolation & purification; Crystallization; Crystallography, X-Ray; Escherichia coli/enzymology; Protein Conformation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.