PMID:10197989
| Citation |
Churchill, JJ, Anderson, DG and Kowalczykowski, SC (1999) The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of chi, resulting in constitutive recombination activation. Genes Dev. 13:901-11 |
|---|---|
| Abstract |
Double-strand DNA break repair and homologous recombination in Escherichia coli proceed by the RecBCD pathway, which is regulated by cis-acting elements known as chi sites. A crucial feature of this regulation is the RecBCD enzyme-directed loading of RecA protein specifically onto the 3'-terminal, chi-containing DNA strand. Here we show that RecBC enzyme (lacking the RecD subunit) loads RecA protein constitutively onto the 3'-terminal DNA strand, with no requirement for chi. This strand is preferentially utilized in homologous pairing reactions. We propose that RecA protein loading is a latent property of the RecBCD holoenzyme, which is normally blocked by the RecD subunit and is revealed following interaction with chi. |
| Links | |
| Keywords |
Blotting, Southern; DNA Repair; DNA, Single-Stranded/metabolism; Escherichia coli/genetics; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases/metabolism; Genes, Bacterial; Models, Genetic; Oligonucleotide Probes; Rec A Recombinases/metabolism; Recombination, Genetic; Time Factors |
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