PMID:10197988
| Citation |
Taylor, AF and Smith, GR (1999) Regulation of homologous recombination: Chi inactivates RecBCD enzyme by disassembly of the three subunits. Genes Dev. 13:890-900 |
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| Abstract |
We report here an unusual mechanism for enzyme regulation: the disassembly of all three subunits of RecBCD enzyme after its interaction with a Chi recombination hot spot. The enzyme, which is essential for the major pathway of recombination in Escherichia coli, acts on linear double-stranded DNA bearing a Chi site to produce single-stranded DNA substrates for strand exchange by RecA protein. We show that after reaction with DNA bearing Chi sites, RecBCD enzyme is inactivated and the three subunits migrate as separate species during glycerol gradient ultracentrifugation or native gel electrophoresis. This Chi-mediated inactivation and disassembly of purified RecBCD enzyme can account for the previously reported Chi-dependent loss of Chi activity in E. coli cells containing broken DNA. Our results support a model of recombination in which Chi regulates one RecBCD enzyme molecule to make a single recombinational exchange ('one enzyme-one exchange' hypothesis). |
| Links | |
| Keywords |
Adenosine Triphosphate/metabolism; Centrifugation, Density Gradient; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Exodeoxyribonuclease V; Exodeoxyribonucleases/metabolism; Exonucleases/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Glycerol/metabolism; Magnesium/pharmacology; Models, Biological; Recombination, Genetic/physiology; Sodium Chloride/chemistry; Time Factors |
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