PMID:10192922
| Citation |
Nagano, K, Wachi, M, Takada, A, Takaku, F, Hirasawa, T and Nagai, K (1999) fcsA29 mutation is an allele of polA gene of Escherichia coli. Biosci. Biotechnol. Biochem. 63:427-9 |
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| Abstract |
The cold-sensitive fcsA29 mutation of Escherichia coli was found to be a new type of cold-sensitive allele of the polA gene encoding DNA polymerase I, caused by an Asp(116)-->Asn change in the 5'-->3' exonuclease domain. The fcsA29 mutant showed typical polA mutant phenotypes such as UV sensitivity and unacceptability of recA mutation. Cold-sensitive growth of the mutant was suppressed by introduction of a sulA mutation, indicating that cell filamentation was due to the SOS response. |
| Links | |
| Keywords |
Alleles; Cell Survival/radiation effects; Cold Temperature; DNA Polymerase I/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Genetic Complementation Test; Point Mutation; Rec A Recombinases/genetics; SOS Response (Genetics)/genetics; Ultraviolet Rays |
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