PMID:10187907
| Citation |
Avaeva, SM, Velichko, TI, Vorobyeva, NN, Kurilova, SA, Nazarova, TI and Sklyankina, VA (1999) Stabilization of the enzyme--substrate complex of the mutant Asp-67Asn inorganic pyrophosphatase from Escherichia coli by fluoride ions. Biochemistry Mosc. 64:169-74 |
|---|---|
| Abstract |
Magnesium-supported PPi hydrolysis by the mutant Asp-67Asn E. coli pyrophosphatase at saturating PPi and metal-activator concentrations in the presence of NaF is followed by a gradual decrease in the initial rate of PPi hydrolysis. The reaction occurs in two steps: first a complex containing enzyme, pyrophosphate, magnesium, and fluoride ions is immediately formed, then its conformation changes slowly. This enzyme--substrate complex stabilized by fluoride is partially active and can be isolated by the removal of excess fluoride by gel-filtration. |
| Links | |
| Keywords |
Aspartic Acid/genetics; Aspartic Acid/metabolism; Enzyme Stability; Escherichia coli/enzymology; Inorganic Pyrophosphatase; Mutagenesis, Site-Directed; Pyrophosphatases/genetics; Pyrophosphatases/metabolism; Sodium Fluoride/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.