PMID:10096080
| Citation |
Barak, R and Eisenbach, M (1999) Chemotactic-like response of Escherichia coli cells lacking the known chemotaxis machinery but containing overexpressed CheY. Mol. Microbiol. 31:1125-37 |
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| Abstract |
We describe a chemotactic-like response of Escherichia coli strains lacking most of the known chemotaxis machinery but containing high levels of the response regulator CheY. The bacteria accumulated in aspartate-containing capillaries, they formed rings on tryptone-containing semisolid agar, and the probability of counterclockwise flagellar rotation transiently increased in response to stimulation with aspartate (10(-10)-10(-5) M; the response was inverted at > 10(-4) M). The temporal response was partial and delayed, as was the response of a control wild-type strain having a high CheY level. alpha-Methyl-DL-aspartate, a non-metabolizable analogue of aspartate as well as other known attractants of E. Coli, glucose and, to a lesser extent, galactose, maltose and serine caused a similar response. So did low concentrations of acetate and benzoate (which, at higher concentrations, act as repellents for wild-type E. coli). Other tested repellents such as indole, Ni2+ and CO2+ increased the clockwise bias. These observations raise the possibility that, at least when the conventional signal transduction components are missing, a non-conventional chemotactic signal transduction pathway might be functional in E. coli. Potential molecular mechanisms are discussed. |
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| Keywords |
Arabinose/pharmacology; Aspartic Acid/pharmacology; Bacterial Proteins; Chemotaxis/physiology; Culture Media; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli/physiology; Membrane Proteins/metabolism; Signal Transduction; Tryptophan/pharmacology |
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