PMID:10096019
| Citation |
Bergstrom, LC, Qin, L, Harlocker, SL, Egger, LA and Inouye, M (1998) Hierarchical and co-operative binding of OmpR to a fusion construct containing the ompC and ompF upstream regulatory sequences of Escherichia coli. Genes Cells 3:777-88 |
|---|---|
| Abstract |
OmpR is a transcription factor that regulates the expression of the porin genes ompF and ompC in Escherichia coli. The phosphorylation state of OmpR, directed by the osmosensor EnvZ, determines its ability to bind to the upstream regulatory regions of these genes, a total of 14 phospho-OmpR binding sites. While it has been possible to study the stoichiometry and hierarchy of the OmpR-DNA interaction in the upstream regions of ompF and ompC, their disunited location on the bacterial chromosome has made it difficult to compare the individual binding affinities of respective sites. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA Footprinting; DNA Mutational Analysis; Densitometry; Electrophoresis/methods; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Regulator; Molecular Sequence Data; Phenanthrolines/chemistry; Phenanthrolines/pharmacology; Porins/drug effects; Porins/genetics; Porins/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Regulatory Sequences, Nucleic Acid; Trans-Activators/genetics; Trans-Activators/metabolism |
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