PMID:10089538
| Citation |
Sol, M, Gomis-Rüth, F, Guasch, A, Serrano, L and Coll, M (1998) Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the receiver domain of PhoB. Acta Crystallogr. D Biol. Crystallogr. 54:1460-3 |
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| Abstract |
PhoB is the response regulator of the E. coli two-component signal transduction system for phosphate regulation. It is a transcription factor that activates more than 30 genes of the pho regulon. Crystals of the receiver domain of PhoB were obtained by applying the hanging-drop vapour-diffusion method. X-ray diffraction data have been collected using synchrotron radiation to 1.88 A resolution. The crystals belong to the orthorhombic space group P212121 with unit-cell constants a = 34.11, b = 60.42, c = 119.97 A. The Matthews parameter suggests that PhoB crystallizes with two molecules per asymmetric unit, suggesting that activating dimerization occurs in the crystal. |
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| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Crystallization; Crystallography, X-Ray; Escherichia coli/chemistry; Escherichia coli/genetics; Membrane Proteins/chemistry; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/isolation & purification; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/isolation & purification |
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