PMID:10089375
| Citation |
Bacchella, L, Lina, C, Todone, F, Negri, A, Tedeschi, G, Ronchi, S and Mattevi, A (1999) Crystallization of L-aspartate oxidase, the first enzyme in the bacterial de novo biosynthesis of NAD. Acta Crystallogr. D Biol. Crystallogr. 55:549-51 |
|---|---|
| Abstract |
The flavoenzyme L-aspartate oxidase from Escherichia coli was crystallized using the hanging-drop vapour-diffusion technique with PEG 4000 as precipitant. The crystals belong to space group P3121 (or P3221) with unit-cell parameters a = b = 84.9, c = 159.9 A. A solvent content of 42% corresponds to a monomer (60 kDa) in the asymmetric unit. A complete 2.8 A resolution data set was collected using a rotating-anode X-ray generator. |
| Links | |
| Keywords |
Amino Acid Oxidoreductases/chemistry; Crystallization; Crystallography, X-Ray; Escherichia coli/enzymology; NAD/biosynthesis |
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