PMID:10080932

Citation	Akaboshi, E (1999) Recognition of sequence-directed structure of the ssDNA backbone by nucleases. Biochem. Biophys. Res. Commun. 256:532-6
Abstract	Escherichia coli endonuclease I and exonuclease VII appear to recognize sequence-dependent conformations in the ssDNA backbone. ssDNAs, containing either A- and/or T-tract or a CAP binding region, were digested with these nucleases under conditions which minimize the formation of secondary structures. The digestion patterns were examined in relation to previous results of biochemical and crystallographic studies on dsDNA, and showed broad agreement. Endonuclease I cleaved ssDNA at sites corresponding to bent sites in dsDNA.
Links	PubMed Online version:10.1006/bbrc.1999.0373
Keywords	Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Cyclic AMP Receptor Protein/metabolism; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/metabolism; Deoxyribonuclease I/metabolism; Escherichia coli/enzymology; Exodeoxyribonucleases/metabolism; Magnesium/pharmacology; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Nucleotides/pharmacology; Poly A/metabolism; Poly T/metabolism; Response Elements/genetics; Substrate Specificity
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