PMID:10075935

Citation	Brandi, A, Spurio, R, Gualerzi, CO and Pon, CL (1999) Massive presence of the Escherichia coli 'major cold-shock protein' CspA under non-stress conditions. EMBO J. 18:1653-9
Abstract	The most characteristic event of cold-shock activation in Escherichia coli is believed to be the de novo synthesis of CspA. We demonstrate, however, that the cellular concentration of this protein is > or = 50 microM during early exponential growth at 37 degrees C; therefore, its designation as a major cold-shock protein is a misnomer. The cspA mRNA level decreases rapidly with increasing cell density, becoming virtually undetectable by mid-to-late exponential growth phase while the CspA level declines, although always remaining clearly detectable. A burst of cspA expression followed by a renewed decline ensues upon dilution of stationary phase cultures with fresh medium. The extent of cold-shock induction of cspA varies as a function of the growth phase, being inversely proportional to the pre-existing level of CspA which suggests feedback autorepression by this protein. Both transcriptional and post-transcriptional controls regulate cspA expression under non-stress conditions; transcription of cspA mRNA is under the antagonistic control of DNA-binding proteins Fis and H-NS both in vivo and in vitro, while its decreased half-life with increasing cell density contributes to its rapid disappearance. The cspA mRNA instability is due to its 5' untranslated leader and is counteracted in vivo by the cold-shock DeaD box RNA helicase (CsdA).
Links	PubMed PMC1171252 Online version:10.1093/emboj/18.6.1653
Keywords	Bacterial Proteins/genetics; Chloramphenicol O-Acetyltransferase/genetics; Cold Temperature; Culture Media; DNA Primers; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/physiology; Feedback; Gene Expression Regulation, Bacterial; Kinetics; Oligonucleotide Probes; RNA Processing, Post-Transcriptional; RNA, Messenger/genetics; RNA, Messenger/metabolism; Time Factors; Transcription Factors/genetics; Transcription, Genetic
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