PMID:10075658
| Citation |
Lillig, CH, Prior, A, Schwenn, JD, Aslund, F, Ritz, D, Vlamis-Gardikas, A and Holmgren, A (1999) New thioredoxins and glutaredoxins as electron donors of 3'-phosphoadenylylsulfate reductase. J. Biol. Chem. 274:7695-8 |
|---|---|
| Abstract |
Reduction of inorganic sulfate to sulfite in prototrophic bacteria occurs with 3'-phosphoadenylylsulfate (PAPS) as substrate for PAPS reductase and is the first step leading to reduced sulfur for cellular biosynthetic reactions. The relative efficiency as reductants of homogeneous highly active PAPS reductase of the newly identified second thioredoxin (Trx2) and glutaredoxins (Grx1, Grx2, Grx3, and a mutant Grx1C14S) was compared with the well known thioredoxin (Trx1) from Escherichia coli. Trx1, Trx2, and Grx1 supported virtually identical rates of sulfite formation with a Vmax ranging from 6.6 units mg-1 (Trx1) to 5.1 units mg-1 (Grx1), whereas Grx1C14S was only marginally active, and Grx2 and Grx3 had no activity. The structural difference between active reductants had no effect upon Km PAPS (22.5 microM). Grx1 effectively replaced Trx1 with essentially identical Km-values: Km trx1 (13.7 microM), Km grx1 (14.9 microM), whereas the Km trx2 was considerably higher (34.2 microM). The results agree with previous in vivo data suggesting that Trx1 or Grx1 is essential for sulfate reduction but not for ribonucleotide reduction in E. coli. |
| Links | |
| Keywords |
Electron Transport; Escherichia coli/metabolism; Glutaredoxins; Kinetics; Oxidoreductases/metabolism; Proteins/metabolism; Ribonucleotides/metabolism; Substrate Specificity; Sulfates/metabolism; Thioredoxins/metabolism |
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