PMID:10050311
| Citation |
Suzuki, T, Imamura, A, Ueguchi, C and Mizuno, T (1998) Histidine-containing phosphotransfer (HPt) signal transducers implicated in His-to-Asp phosphorelay in Arabidopsis. Plant Cell Physiol. 39:1258-68 |
|---|---|
| Abstract |
His to Asp phosphorelay signal transduction mechanisms involve three types of widespread signaling components: a sensor His-kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) domain. In Arabidopsis, several sensor His-kinases have recently been discovered (e.g., ETR1 and CKI1) through extensive genetic studies. Furthermore, a recent search for response regulators in this higher plant revealed that it possesses a group of response regulators (ARR-series), each of which exhibits the phospho-accepting receiver function. However, no signal transducer containing the HPt domain has been reported. Here we identify three distinct Arabidopsis genes (AHP1 to AHP3), each encoding a signal transducer containing a HPt domain. Both in vivo and in vitro evidence that each AHP can function as a phospho-transmitting HPt domain with an active histidine site was obtained by employing both the Escherichia coli and yeast His-Asp phosphorelay systems. It was demonstrated that AHP1 exhibits in vivo ability to complement a mutational lesion of the yeast YPD1 gene, encoding a typical HPt domain involved in an osmosensing signal transduction. It was also demonstrated that AHPs can interact in vitro with ARRs through the His-Asp phosphotransfer reaction. It was thus suggested that the uncovered sensors-AHPs-ARRs lineups may play important roles in propagating environmental stimuli through the multistep His-Asp phosphorelay in Arabidopsis. |
| Links | |
| Keywords |
Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis Proteins; Aspartic Acid/metabolism; Catalytic Domain; Chromosome Mapping; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Fungal Proteins/genetics; Genes, Plant; Genetic Complementation Test; Histidine/metabolism; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Phosphotransferases/genetics; Plant Proteins/genetics; Protein Kinases/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Signal Transduction/genetics |
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