PMID:10049386
| Citation |
Kunte, HJ, Crane, RA, Culham, DE, Richmond, D and Wood, JM (1999) Protein ProQ influences osmotic activation of compatible solute transporter ProP in Escherichia coli K-12. J. Bacteriol. 181:1537-43 |
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| Abstract |
ProP is an osmoregulatory compatible solute transporter in Escherichia coli K-12. Mutation proQ220::Tn5 decreased the rate constant for and the extent of ProP activation by an osmotic upshift but did not alter proP transcription or the ProP protein level. Allele proQ220::Tn5 was isolated, and the proQ sequence was determined. Locus proQ is upstream from prc (tsp) at 41.2 centisomes on the genetic map. The proQ220::Tn5 and prc phenotypes were different, however. Gene proQ is predicted to encode a 232-amino-acid, basic, hydrophilic protein (molecular mass, 25,876 Da; calculated isoelectric point, 9.66; 32% D, E, R, or K; 54.5% polar amino acids). The insertion of PCR-amplified proQ into vector pBAD24 produced a plasmid containing the wild-type proQ open reading frame, the expression of which yielded a soluble protein with an apparent molecular mass of 30 kDa. Antibodies raised against the overexpressed ProQ protein detected cross-reactive material in proQ+ bacteria but not in proQ220::Tn5 bacteria. ProQ may be a structural element that influences the osmotic activation of ProP at a posttranslational level. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Membrane/physiology; Chromosome Mapping; Conserved Sequence; DNA Transposable Elements; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Membrane Transport Proteins; Molecular Sequence Data; Mutagenesis, Insertional; Osmolar Concentration; Proline/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Sodium Chloride/pharmacology; Subcellular Fractions/metabolism; Symporters |
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