PMID:10049308

Citation	Cheung, MS, Daizadeh, I, Stuchebrukhov, AA and Heelis, PF (1999) Pathways of electron transfer in Escherichia coli DNA photolyase: Trp306 to FADH. Biophys. J. 76:1241-9
Abstract	We describe the results of a series of theoretical calculations of electron transfer pathways between Trp306 and *FADH. in the Escherichia coli DNA photolyase molecule, using the method of interatomic tunneling currents. It is found that there are two conformationally orthogonal tryptophans, Trp359 and Trp382, between donor and acceptor that play a crucial role in the pathways of the electron transfer process. The pathways depend vitally on the aromaticity of tryptophans and the flavin molecule. The results of this calculation suggest that the major pathway of the electron transfer is due to a set of overlapping orthogonal pi-rings, which starts from the donor Trp306, runs through Trp359 and Trp382, and finally reaches the flavin group of the acceptor complex, FADH.
Links	PubMed PMC1300104 Online version:10.1016/S0006-3495(99)77287-5
Keywords	Binding Sites; Biophysical Phenomena; Biophysics; DNA Repair; Deoxyribodipyrimidine Photo-Lyase/chemistry; Deoxyribodipyrimidine Photo-Lyase/metabolism; Electron Transport; Escherichia coli/enzymology; Flavin-Adenine Dinucleotide/metabolism; Models, Molecular; Protein Conformation; Pyrimidine Dimers/metabolism; Pyrimidine Dimers/radiation effects; Thermodynamics; Tryptophan/chemistry
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