PMID:10048032
| Citation |
Brocklehurst, KR, Hobman, JL, Lawley, B, Blank, L, Marshall, SJ, Brown, NL and Morby, AP (1999) ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli. Mol. Microbiol. 31:893-902 |
|---|---|
| Abstract |
We have identified the promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance (mer) operons, including a long spacer region containing an inverted repeat sequence. The gene encoding the transcriptional regulator of PzntA, designated zntR, has been identified from genome sequence data, by expression of the gene product and by insertional inactivation/complementation. The ZntR product is a member of the MerR family of transcriptional regulators and appears to act as a hypersensitive transcriptional switch. A hybrid MerR/ZntR protein has been constructed and indicates that the C-terminal region of ZntR recognizes Zn(II). |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/physiology; Bacterial Proteins; Dose-Response Relationship, Drug; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Regulator; Neural Cell Adhesion Molecules/genetics; Promoter Regions, Genetic/genetics; Receptor Protein-Tyrosine Kinases/genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transcription Factors/genetics; Zinc/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
