PMID:10047479
| Citation |
McClain, WH, Gabriel, K, Bhattacharya, S, Jou, YY and Schneider, J (1999) Functional compensation by particular nucleotide substitutions of a critical G*U wobble base-pair during aminoacylation of transfer RNA. J. Mol. Biol. 286:1025-32 |
|---|---|
| Abstract |
Expression of the genetic code depends on precise tRNA aminoacylation by cognate aminoacyl-tRNA synthetase enzymes. The G.U wobble base-pair in the acceptor helix of Escherichia coli alanine tRNA is the primary aminoacylation determinant of this molecule. Previous work on the process of synthetase recognition of the G.U pair showed that replacing G.U by a G.C Watson-Crick base-pair inactivates alanine acceptance by the tRNA, but that C.A and G.A wobble pair replacements preserve acceptance. Work by another group reported that the effects of a G.C replacement were reversed by a distal wobble base-pair in the anticodon helix. This result is potentially interesting because it suggests that distant regions in alanine tRNA are functionally coupled during synthetase recognition and more generally because recognition determinants of many other tRNAs lie in both the acceptor helix and anticodon helix region. Here, we have conducted an extensive in vivo analysis of the distal wobble pair in alanine tRNA and report that it does not behave like a compensating mutation. Restoration of alanine acceptance was not detected even when the synthetase enzyme was overproduced. We discuss the previous experimental evidence and suggest how the distal wobble pair was incorrectly analyzed. The available data indicate that all principal recognition determinants of alanine tRNA lie in the molecule's acceptor helix. |
| Links |
PubMed Online version:10.1006/jmbi.1999.2542 |
| Keywords |
Acylation; Alanine/chemistry; Amino Acyl-tRNA Synthetases/metabolism; Base Pairing; Base Sequence; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Guanine/chemistry; Molecular Sequence Data; Nucleic Acid Conformation; Point Mutation; RNA, Transfer, Amino Acyl/chemistry; RNA, Transfer, Amino Acyl/genetics; RNA, Transfer, Amino Acyl/metabolism; Uridine/chemistry |
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