PMID:10027987
| Citation |
Ghigo, JM, Weiss, DS, Chen, JC, Yarrow, JC and Beckwith, J (1999) Localization of FtsL to the Escherichia coli septal ring. Mol. Microbiol. 31:725-37 |
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| Abstract |
In Escherichia coli, nine gene products are known to be essential for assembly of the division septum. One of these, FtsL, is a bitopic membrane protein whose precise function is not understood. Here we use fluorescence microscopy to study the subcellular localization of FtsL, both in a wild-type strain and in a merodiploid strain that expresses a GFP-FtsL fusion protein. We show that FtsL localizes to the cell septum where it forms a ring analogous to the cytoplasmic FtsZ ring. FtsL localization is dependent upon the function of FtsZ, FtsA and FtsQ, but not FtsI. In a reverse approach, we use fusions of green fluorescent protein (GFP) to FtsZ, FtsA and ZipA to show that these proteins localize to the division site in an FtsL-independent fashion. We propose that FtsL is a relatively late recruit to the ring structure that mediates septation. |
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| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cytoskeletal Proteins; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Green Fluorescent Proteins; Hexosyltransferases/genetics; Hexosyltransferases/metabolism; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Microscopy, Fluorescence; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Muramoylpentapeptide Carboxypeptidase; Penicillin-Binding Proteins; Peptidoglycan Glycosyltransferase; Peptidyl Transferases/genetics; Peptidyl Transferases/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
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