|Genotype||mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC8-2) ΩPN25-hsdS (CC8-1)|
This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 8 – NRS384) were introduced onto the chromosome at neutral locations via recombineering.
The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation.
Plasmids isolated from this E. coli strain transform S. aureus clonal complex 8 – NRS384 at high efficiency.