Category:Complex:1-deoxyxylulose-5-phosphate synthase
About
Description (originally from EcoCyc[1]) 1-deoxyxylulose-5-phosphate synthase
Comments (originally from EcoCyc[1]) 1-deoxyxylulose-5-phosphate synthase (Dxs) catalyzes the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, as well as feeding into the pyridoxal 5-phosphate and thiamine biosynthesis pathways.
Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [2][3][3][4][5][6][7][8][9][10][11][12].
References
- ↑ 1.0 1.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Sprenger, GA et al. (1997) Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol. Proc. Natl. Acad. Sci. U.S.A. 94 12857-62 PubMed
- ↑ 3.0 3.1 Lois, LM et al. (1998) Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 95 2105-10 PubMed
- ↑ Lawhorn, BG et al. (2004) A genetic screen for the identification of thiamin metabolic genes. J. Biol. Chem. 279 43555-9 PubMed
- ↑ Begley, TP et al. (1999) Thiamin biosynthesis in prokaryotes. Arch. Microbiol. 171 293-300 PubMed
- ↑ Matthews, PD & Wurtzel, ET (2000) Metabolic engineering of carotenoid accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase. Appl. Microbiol. Biotechnol. 53 396-400 PubMed
- ↑ Alper, H et al. (2005) Tuning genetic control through promoter engineering. Proc. Natl. Acad. Sci. U.S.A. 102 12678-83 PubMed
- ↑ Kim, SW & Keasling, JD (2001) Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol. Bioeng. 72 408-15 PubMed
- ↑ Jones, KL et al. (2000) Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria. Metab. Eng. 2 328-38 PubMed
- ↑ Xiang, S et al. (2007) Crystal structure of 1-deoxy-D-xylulose 5-phosphate synthase, a crucial enzyme for isoprenoids biosynthesis. J. Biol. Chem. 282 2676-82 PubMed
- ↑ Querol, J et al. (2001) A fluorometric assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase activity. Anal. Biochem. 296 101-5 PubMed
- ↑ Han, YS et al. (2003) High-performance liquid chromatography assay for 1-deoxy-D-xylulose 5-phosphate synthase activity using fluorescence detection. J Chromatogr A 986 291-6 PubMed
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