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PMID:3526348
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| Citation |
Tabor, CW, Tabor, H and Xie, QW (1986) Spermidine synthase of Escherichia coli: localization of the speE gene. Proc. Natl. Acad. Sci. U.S.A. 83:6040-4 |
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| Abstract |
We have obtained Escherichia coli mutants lacking spermidine synthase (putrescine aminopropyltransferase) and have found that the mutated gene (speE) is located immediately upstream from the gene coding for S-adenosylmethionine decarboxylase (speD); these genes are located at 2.7 minutes on the E. coli chromosome. Both genes are present in a 1795-base-pair fragment of E. coli DNA that was cloned into pBR322. Deletion of 105 bases upstream of speE caused a coordinate loss of both activities, indicating that speE and speD constitute a single operon. speE and speD have also been cloned separately in a high-expression vector; strains carrying these plasmids overproduce the respective enzymes. |
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| Keywords |
Adenosylmethionine Decarboxylase/genetics; Chromosome Deletion; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Genotype; Operon; Plasmids; Species Specificity; Spermidine Synthase/genetics; Transferases/genetics |
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