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...arimova, G, Pidoux, J, Ullmann, A and Ladant, D''' (1998) A bacterial two-hybrid system based on a reconstituted signal transduction pathway. ''Proc. Natl. ...her for ligands that bind to a given "bait," as in the classical yeast two-hybrid system, or for molecules or mutations that block a given interaction betwee ...

...rotein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters ...365 mV for the three redox transitions). The physiological function of the hybrid-cluster protein has not yet been elucidated. The protein is only detected i ...

...glycan glycosyltransferase (MtgA) catalyzes glycan chain elongation of the bacterial cell wall. Here we show that MtgA localizes at the division site of Escheri ...ptidase/genetics; Serine-Type D-Ala-D-Ala Carboxypeptidase/metabolism; Two-Hybrid System Techniques ...

...e, to eliminate intragenomic crossovers between homologous segments of the bacterial chromosome that might otherwise lead to deleterious inversions or deletions Bacterial Proteins/metabolism; DNA Helicases; DNA, Viral/metabolism; DNA, Viral/ultra ...

...the pyruvate dehydrogenase complex genes and gene-DNA relationships in the 2 to 3 minute region of the Escherichia coli chromosome. ''J. Gen. Microbiol. ...construct a 47-site (15 enzymes) restriction map for a 24.2 kb segment of bacterial DNA in the nadC-lpd region. A further 13 sites (six enzymes) were defined i ...

...asured turnover rates of up to 0.7 min-1. All cleavage sites were at least 2 nt away from the 5' and 3' ends of the oligonucleotides. Some cleavage site ...ism; Kinetics; Oligonucleotides/metabolism; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; RNA, Catalytic/metabolism; Ribonuclease P; Substrate Specificit ...

'''Galli, E and Gerdes, K''' (2010) Spatial resolution of two bacterial cell division proteins: ZapA recruits ZapB to the inner face of the Z-ring. .... A direct interaction between ZapA and ZapB is supported by bacterial two-hybrid and in vitro interaction assays. Using high-resolution 3-D reconstruction m ...

...four tricarboxylic acid cycle genes are contained in a 12.8-kb segment of bacterial DNA. The four gene products, plus a possible succinate dehydrogenase small ...ar; DNA Restriction Enzymes; DNA, Viral; Escherichia coli/genetics; Genes, Bacterial; Ketoglutarate Dehydrogenase Complex/genetics; Succinate Dehydrogenase/gene ...

...' (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus ...otein. GcpE was shown to interact strongly with PetF via the bacterial two-hybrid system designed to detect protein-protein interactions. Moreover, a direct ...

...somes: structure of YaeJ bound to the ribosome. ''Science'' '''335''':1370-2 ...cuing stalled ribosomes in a tmRNA-independent manner. Here, we report a 3.2 angstrom-resolution crystal structure of the rescue factor YaeJ bound to th ...

...hysical interactions between these replication initiators by bacterial two-hybrid and pull-down/Western blot assays, lead us to propose the possibility that == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...ed to total impairment of beta' binding. In vitro, FecI was retained on Ni(2+)-nitrilotriacetic acid agarose loaded with a His-tagged beta'(1-313) fragm ...igma Factor/genetics; Sigma Factor/metabolism; Transcription, Genetic; Two-Hybrid System Techniques ...

...alpha-subunit that represents a step in catalysis. Many lines of evidence (hybrid enzyme formation, oxygen exchange kinetics, 31P-n.m.r. studies) suggest tha == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...(1993) Structural and evolutionary relationships between two families of bacterial extracytoplasmic chaperone proteins which function cooperatively in fimbria ...l as a functional unit. Two exceptions were noted, however. In one case, a hybrid protein appears to have arisen, possibly by genetic recombination. In anoth ...

...iency: the P1 promoter of pBR322 and a hybrid promoter which contains both bacterial and pBR322 DNA sequences. ...lar; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Genes, Regulator; Glycerol Kinase/genetics; Molecular Sequence Data; Phosp ...

...fusion to the amino terminus of gp64. A general expression vector, pAcSurf-2, was constructed in which multiple cloning sites were positioned in-phase b == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...