PMID:5073742
| Citation |
Brockes, JP, Brown, PR and Murray, K (1972) The deoxyribonucleic acid modification enzyme of bacteriophage P1. Biochem. J. 127:1-10 |
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| Abstract |
The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20-24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0-6.25: the apparent K(m) for S-adenosyl-l-methionine is 5x10(-6)m. |
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| Keywords |
Ammonium Sulfate; Catalase; Centrifugation, Zonal; Chromatography, DEAE-Cellulose; Coliphages/enzymology; DNA, Viral; Dialysis; Hydrogen-Ion Concentration; Hydrolysis; Methylation; Methyltransferases/isolation & purification; Morpholines; Purines; S-Adenosylmethionine; Sulfonic Acids; Tritium |
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