SG4121

Name	SG4121
Synonyms
Genotype	lon-100 Δ(gal-pgl)324 lac thi rpsL recA hsdR
Phenotype	Streptomycin resistant, non-mucoid, Lon-
Sources	LMBP 671
References	PMID:1312934

Notes

The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688).
In addition, this strain is K12 restriction negative / modification positive, and RecA deficient.