PMID:21998391
| Citation |
Yeeles, JT and Marians, KJ (2011) The Escherichia coli replisome is inherently DNA damage tolerant. Science 334:235-8 |
|---|---|
| Abstract |
The Escherichia coli DNA replication machinery must frequently overcome template lesions under normal growth conditions. Yet, the outcome of a collision between the replisome and a leading-strand template lesion remains poorly understood. Here, we demonstrate that a single, site-specific, cyclobutane pyrimidine dimer leading-strand template lesion provides only a transient block to fork progression in vitro. The replisome remains stably associated with the fork after collision with the lesion. Leading-strand synthesis is then reinitiated downstream of the damage in a reaction that is dependent on the primase, DnaG, but independent of any of the known replication-restart proteins. These observations reveal that the replisome can tolerate leading-strand template lesions without dissociating by synthesizing the leading strand discontinuously. |
| Links |
PubMed PMC3593629 Online version:10.1126/science.1209111 |
| Keywords |
DNA Damage; DNA Polymerase III/metabolism; DNA Replication; DNA, Bacterial/biosynthesis; DNA-Directed DNA Polymerase/metabolism; Endodeoxyribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Exodeoxyribonucleases/metabolism; Multienzyme Complexes/metabolism; Plasmids; Pyrimidine Dimers; Replication Origin; Templates, Genetic |
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