PMID:2183003
| Citation |
Ogura, T, Niki, H, Kano, Y, Imamoto, F and Hiraga, S (1990) Maintenance of plasmids in HU and IHF mutants of Escherichia coli. Mol. Gen. Genet. 220:197-203 |
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| Abstract |
Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the delta hupA-delta hupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the delta hupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the delta hupA-delta hupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacteriocin Plasmids; Base Sequence; DNA-Binding Proteins/genetics; Escherichia coli/genetics; F Factor; Genes, Bacterial; Genetic Complementation Test; Integration Host Factors; Molecular Sequence Data; Mutation; Plasmids; Restriction Mapping; Time Factors; Transformation, Bacterial |
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