PMID:2180922
| Citation |
Pierson, DE and Campbell, A (1990) Cloning and nucleotide sequence of bisC, the structural gene for biotin sulfoxide reductase in Escherichia coli. J. Bacteriol. 172:2194-8 |
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| Abstract |
Clones of the Escherichia coli bisC locus have been isolated by complementing a bisC mutant for growth with d-biotin d-sulfoxide as a biotin source. The complementation properties of deletions and Tn5 insertions located the bisC gene to a 3.7-kilobase-pair (kbp) segment, 3.3 kbp of which has been sequenced. A single open reading frame of 2,178 bp, capable of encoding a polypeptide of molecular weight 80,905, was found. In vitro transcription of plasmids carrying the wild-type sequence and deletion and insertion mutants showed that BisC complementation correlated perfectly with production of a polypeptide whose measured molecular weight (79,000) does not differ significantly from 80,905. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Molecular Sequence Data; Mutation; Oxidoreductases/genetics; Restriction Mapping |
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