PMID:2681148
| Citation |
No author listed (1989) The tyrT locus of Escherichia coli exhibits a regulatory function for glycine metabolism. J. Bacteriol. 171:5987-94 |
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| Abstract |
The tyrT locus in Escherichia coli codes for two gene copies of tRNA(1Tyr). Both genes are organized in one operon, which has a unique structure. The two tRNA genes are separated by a spacer segment highly homologous to a part of a unit which is repeated three times in the distal portion of the locus. This operon also contains coding capacity for a small basic protein. A genomic deletion of this locus was constructed and marked by a kanamycin resistance cassette. Deletion mutants exhibited a characteristic phenotype when cells were shifted from rich medium to minimal medium. The cells entered a transient lag phase, apparently resulting from specific glycine starvation. This phenotype involved stringent response and was therefore not observed in relA derivatives. The genomic deletion was complemented in trans by a plasmid-borne tyrT locus. From deletion mapping, it can be concluded that a product of the tyrT operon is responsible for complementation. However, neither the tRNA(1Tyr) nor the proposed basic protein is the complementation-competent entity. |
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| Keywords |
Amino Acids/metabolism; Blotting, Southern; Chromosome Deletion; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes, Bacterial; Genes, Regulator; Genetic Complementation Test; Genotype; Glycine/metabolism; Mutation; Nucleic Acid Hybridization; RNA, Transfer, Amino Acid-Specific/genetics; RNA, Transfer, Tyr/genetics; Restriction Mapping |
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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