PMID:21402902

Citation	No author listed (2011) Direct regulation of Escherichia coli ribosomal protein promoters by the transcription factors ppGpp and DksA. Proc. Natl. Acad. Sci. U.S.A. 108:5712-7
Abstract	We show here that the promoters for many of the Escherichia coli ribosomal protein operons are regulated directly by two transcription factors, the small RNA polymerase-binding protein DksA and the nutritional stress-induced nucleotide ppGpp. ppGpp and DksA work together to inhibit transcription initiation from ribosomal protein promoters in vitro and in vivo. The degree of promoter regulation by ppGpp/DksA varies among the r-protein promoters, but some are inhibited almost as much as rRNA promoters. Thus, many r-protein operons are regulated at the level of transcription in addition to their control by the classic translational feedback systems discovered ~30 y ago. We conclude that direct control of r-protein promoters and rRNA promoters by the same signal, ppGpp/DksA, makes a major contribution to the balanced and coordinated synthesis rates of all of the ribosomal components.
Links	PubMed PMC3078377 Online version:10.1073/pnas.1019383108
Keywords	Escherichia coli/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial/genetics; Plasmids/genetics; Promoter Regions, Genetic/genetics; Pyrophosphatases/metabolism; Ribosomal Proteins/genetics; Transcription Factors/metabolism; beta-Galactosidase
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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