Phage P1
Bacteriophage P1 Phage P1 was isolated in 1951 by Giuseppe Bertani from the Escherichia coli strain of Lisbonne and Carrere. [1]. P1 has been widely used to construct new batcterial strains and was used extensively to map the Escherichia Coli chromosome. P1 has served as a model organism for different aspects of phage and biology such as DNA restriction modification[2][3],site-specific recombination[4], plasmid replication[5], partition[6], incompatibility[7] and addiction[8]
Morphology
Phage P1 exhibits the classical bacteriophage morphology with an icosahedral head, 220nm long inflexible tail with a complete tube surrounded by a contractile sheath, baseplate and six kinked tail fibers. The icosahedral head contains the phage genome. A variable part(encoded by an invertible segment of P1 DNA) of the tail fibers(1 to 2nm thick) determines the specificity of P1 adsorption on different hosts.[9]
Genome Organization
AF234172.1 on NCBI AF234173.1 on NCBI
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Life cycle
Lytic cycle
Adsorbtion and Injection
Early gene expression
Middle gene expression
DNA replication
Late gene expression
Packaging
Lysis
Lysogenic state
Lysis-Lysogeny switch
Prophage induction
See also
- Phage P1 Gene List
- NC 005856 on NCBI
- GenBank record for P1
- Phage P1 Taxonomy on NCBI
- International Committee on Taxonomy of Viruses
- Wikipedia: Phage P1
References
See Help:References for how to manage references in EcoliWiki.
- ↑ BERTANI, G (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62 293-300 PubMed
- ↑ ARBER, W & DUSSOIX, D (1962) Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage lambda. J. Mol. Biol. 5 18-36 PubMed
- ↑ DUSSOIX, D & ARBER, W (1962) Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage lambda. J. Mol. Biol. 5 37-49 PubMed
- ↑ Hamilton, DL & Abremski, K (1984) Site-specific recombination by the bacteriophage P1 lox-Cre system. Cre-mediated synapsis of two lox sites. J. Mol. Biol. 178 481-6 PubMed
- ↑ Chattoraj, DK (2000) Control of plasmid DNA replication by iterons: no longer paradoxical. Mol. Microbiol. 37 467-76 PubMed
- ↑ Austin, S & Abeles, A (1983) Partition of unit-copy miniplasmids to daughter cells. I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition. J. Mol. Biol. 169 353-72 PubMed
- ↑ Sternberg, N et al. (1981) Group Y incompatibility and copy control of P1 prophage. Plasmid 5 138-49 PubMed
- ↑ Lehnherr, H et al. (1993) Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained. J. Mol. Biol. 233 414-28 PubMed
- ↑ Łobocka, MB et al. (2004) Genome of bacteriophage P1. J. Bacteriol. 186 7032-68 PubMed