SMD Annotating Batch Files
As of Jan 22, 2010, Debby & I (BM) have decided on the following list of potential terms for the key word in the c-4 annotation column for SMD.
- The most important thing is KEY word! Pick the term that BEST suits the sample.
- Annotations are for each sample in an experiment set. The term chosen may vary accordingly in the set, but each sample will be given a term.
Conventions for the <c-1> field: Name of the strain
E. coli O157-H7 str. xxx
E. coli K-12 xxx
Conventions for the <c-3> field: Describe medium, growth temperature, chemicals added, etc.
37C
biofilm
planktonic
glass wool biofilm
uM for micromolar
ug for microgram
MOPS minimal medium + carbon source
time course
SMD field <c-4> terms
| Potential c-4 terms | Example or brief explanation |
|---|---|
|
Biofilm vs planktonic |
|
|
Biofilm + chemical |
Put the actual chemical name in. |
|
Chemical |
Again, put the chemical name here. |
|
Control |
ie. if sample is replicate 1 vs replicate 2 of same strain and same growth conditions |
|
Ultraviolet (UV) light |
|
|
Wild type vs mutant |
|
|
Wild type vs complemented null mutant |
|
|
Wild type vs overexpression |
|
|
Null mutant vs complemented null mutant |
|
|
Strain comparison |
ie. E. coli K-12 MG1655 compared to E. coli B REL606 |
|
Stationary phase |
This includes starvation. |
|
Heat shock |
|
|
Cold shock |
|
|
Minimal medium + carbon source |
|
|
Mutant |
|
|
Growth curve |
Following expression changes as a function of cell density. |
|
Parental strain |
|
|
Aerobic growth |
|
|
Anaerobic growth |
|
|
No treatment |
|
| edit table |