PMID:378999
Rock, CO and Cronan, JE (1979) Solubilization, purification, and salt activation of acyl-acyl carrier protein synthetase from Escherichia coli.J. Biol. Chem. 254:7116-22
| Abstract | No abstract in PubMed |
| Links | PubMed |
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016021 |
integral to membrane |
IDA: Inferred from Direct Assay |
C |
Aas was isolated from the pellet of a centrifugation performed after mechanical disruption of the cells at 80,000 x g for 90 min and several salt washes (pp. 7116). |
complete | |||
| GO:0008779 |
acyl-(acyl-carrier-protein)-phospholipid O-acyltransferase activity |
IDA: Inferred from Direct Assay |
F |
Purified acyl-ACP synthetase was incubated in a reaction with radiolabeled fatty acid (C16:0). THe mixture was filtered and the filter washed with chloroform:methanol:acetic acid to remove free radiolabeled fatty acid. The remaining radiolabeled product (C16:0-ACP) was then measured (pp.7116-7117, Table 2). |
complete | |||
| GO:0008922 |
long-chain-fatty-acid-(acyl-carrier-protein) ligase activity |
IDA: Inferred from Direct Assay |
F |
Purified acyl-ACP synthetase was incubated in a reaction with radiolabeled fatty acid (C16:0). THe mixture was filtered and the filter washed with chloroform:methanol:acetic acid to remove free radiolabeled fatty acid. The remaining radiolabeled product (C16:0-ACP) was then measured (pp.7116-7117, Table 2). |
complete | |||
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