Bicyclomycin
Bicyclomycin, discovered in 1972, has a structure that is unique among antibiotics. Currently used to treat nonspecific diarrhea, it has a low toxicity, and no cross-resistance with other antibiotics has yet been found. It is effective against many Gram-negative bacteria. Bicyclomycin is also effective against the Gram-positive bacterium Micrococcus luteus.[1] It is the only known selective inhibitor of Rho.
Mode of Action
Bicyclomycin inhibits Rho by being a reversible, noncompetitive inhibitor of ATP.[1]
Structure
Targets
Bicyclomycin targets the transcription termination factor Rho.[2]
Mechanisms of Resistance
Mutations that affect sensitivity
Genes in E. coli that affect sensitivity to bicyclomycin.[3]
| Gene | Allele | Sensitivity to BCM | Reference |
|---|---|---|---|
|
recA |
deletion |
increased senstivity |
|
|
recO |
deletion |
increased sensitivity |
|
|
rdgC |
deletion |
increased sensitivity |
|
|
trxA |
deletion |
increased sensitivity |
|
|
trxB |
deletion |
increased sensitivity |
|
|
minC |
deletion |
increased sensitivity |
|
|
envC |
deletion |
increased sensitivity |
|
|
csgG |
deletion |
increased sensitivity |
|
|
pgpB |
deletion |
increased sensitivity |
|
|
yciB |
deletion |
increased sensitivity |
|
|
ydcS |
deletion |
increased sensitivity |
|
|
ydcX |
deletion |
increased sensitivity |
|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Kohn, H & Widger, W (2005) The molecular basis for the mode of action of bicyclomycin. Curr Drug Targets Infect Disord 5 273-95 PubMed
- ↑ Zwiefka, A et al. (1993) Transcription termination factor rho: the site of bicyclomycin inhibition in Escherichia coli. Biochemistry 32 3564-70 PubMed
- ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed