yafY:Quickview: Difference between revisions
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yfjS and yafY are homologs. The first 17 aa of YafY are a type II signal peptide (Matsuyama, unpublished, cited in Miyadai, 2004). Although overproduction of YafY induces degP expression, YjfS does not; chimeric protein experiments demonstrate that the N- and C- termini of YafY have the degP stimulatory determinants (Miyadai, 2004). Originally, the annotation of yafY was fused to the upstream pseudogene ykfK. It is possible that a YkfK-YafW chimeric pseudogene product is made in vivo, but no function would be expected since cleavage of the type II signal is likely to be blocked in any chimeric protein synthesized. Expression of the native YafY lipoprotein gene in the chromosome has not yet been reported. E. coli B has a third protein in this family (UniProt:A2UFU7), 55% identical to YafY and YfjS, that demonstrates conservation of the shorter ORFs and their lipoprotein signals in this novel prophage gene family.<ref name='LIB:EcoGene'/> | |||
Revision as of 02:58, 3 March 2009
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| References | Suggestions |
| Gene Name | |
|---|---|
| Product(s) |
YafY product[1]: CP4-6 prophage; predicted DNA-binding transcriptional regulator product, B0251 product |
| Description | |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity |
|
| edit table |
See Help:Quickview for help with entering information in the Quickview table.
Notes
yfjS and yafY are homologs. The first 17 aa of YafY are a type II signal peptide (Matsuyama, unpublished, cited in Miyadai, 2004). Although overproduction of YafY induces degP expression, YjfS does not; chimeric protein experiments demonstrate that the N- and C- termini of YafY have the degP stimulatory determinants (Miyadai, 2004). Originally, the annotation of yafY was fused to the upstream pseudogene ykfK. It is possible that a YkfK-YafW chimeric pseudogene product is made in vivo, but no function would be expected since cleavage of the type II signal is likely to be blocked in any chimeric protein synthesized. Expression of the native YafY lipoprotein gene in the chromosome has not yet been reported. E. coli B has a third protein in this family (UniProt:A2UFU7), 55% identical to YafY and YfjS, that demonstrates conservation of the shorter ORFs and their lipoprotein signals in this novel prophage gene family.[4]
References
- ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.