PMID:16858726: Difference between revisions
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | !|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
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TpiA | TpiA | ||
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[http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737] | [[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737] | ||
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cytoplasm | cytoplasm | ||
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complete | complete | ||
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[[mscL:Gene_Product(s)|MscL]] | |||
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[[Category:GO:0005624_!_membrane_fraction]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005624_!_membrane_fraction GO:0005624] | |||
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membrane fraction | |||
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IDA: Inferred from Direct Assay | |||
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C | |||
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complete | |||
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Revision as of 17:58, 6 February 2009
Lasserre, JP, Beyne, E, Pyndiah, S, Lapaillerie, D, Claverol, S and Bonneu, M (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis.Electrophoresis 27:3306-21
| Abstract | Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of Escherichia coli were separated. Then, the different partners of each protein complex were identified by LC-MS/MS. In this report, 306 protein complexes were separated and identified. Among these protein complexes, 50 heteromultimeric and 256 homomultimeric protein complexes were found. Among the 50 heteromultimeric protein complexes, 18 previously described protein complexes validate the technology. In this study, 109 new protein complexes were found, providing insight into the function of previously uncharacterized bacterial proteins. |
| Links | PubMed |
Significance
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
TpiA |
GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
Supplemental information, row 163 of table. |
complete | ||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
complete | ||||
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