PMID:3531165

Crowell, DN, Anderson, MS and Raetz, CR (1986) Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.J. Bacteriol. 168:152-9

Abstract

Several enzymes have been discovered recently in crude extracts of Escherichia coli that appear to be involved in the biosynthesis of the lipid A component of lipopolysaccharide. Two of these are lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase. Lipid A disaccharide synthase activity is barely detectable in cells harboring a lesion in the lpxB (pgsB) gene. We subcloned the lpxB gene from plasmid pLC26-43 of the Clarke and Carbon collection (L. Clarke and J. Carbon, Cell 9:91-99, 1976) and localized it to a 1.7-kilobase-pair fragment of DNA counterclockwise of dnaE on the E. coli chromosome. Furthermore, we discovered a new gene (lpxA) located adjacent to and counterclockwise of lpxB that encodes or controls UDP-N-acetylglucosamine acyltransferase. Our data prove that lpxB and lpxA are transcribed in the clockwise direction and suggest that they may be cotranscribed.

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Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
LpxA and LpxB		GO:0009245	lipid A biosynthetic process	IEP: Inferred from Expression Pattern		P	Expression of genes on a DNA fragment derived from pLC25-43 from the Clarke-Carbon collection (PMID:788919) was found to result in the accumulation of Lipid A disaccharide synthase (LpxB) and UDP-N-acetylglucosamine acyltransferase (LpxA) activity (Table 2).	complete
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