PMID:3531165: Difference between revisions
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | !|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
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LpxA and LpxB | |||
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[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245] | |||
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lipid A biosynthetic process | |||
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IEP: Inferred from Expression Pattern | |||
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P | |||
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Expression of genes on a DNA fragment derived from pLC25-43 from the Clarke-Carbon collection (PMID:???) was found to result in the accumulation of Lipid A disaccharide synthase (LpxB) and UDP-N-acetylglucosamine acyltransferase (LpxA) activity (Table 2). | |||
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complete | |||
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Revision as of 16:51, 27 October 2008
Crowell, DN, Anderson, MS and Raetz, CR (1986) Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.J. Bacteriol. 168:152-9
| Abstract | Several enzymes have been discovered recently in crude extracts of Escherichia coli that appear to be involved in the biosynthesis of the lipid A component of lipopolysaccharide. Two of these are lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase. Lipid A disaccharide synthase activity is barely detectable in cells harboring a lesion in the lpxB (pgsB) gene. We subcloned the lpxB gene from plasmid pLC26-43 of the Clarke and Carbon collection (L. Clarke and J. Carbon, Cell 9:91-99, 1976) and localized it to a 1.7-kilobase-pair fragment of DNA counterclockwise of dnaE on the E. coli chromosome. Furthermore, we discovered a new gene (lpxA) located adjacent to and counterclockwise of lpxB that encodes or controls UDP-N-acetylglucosamine acyltransferase. Our data prove that lpxB and lpxA are transcribed in the clockwise direction and suggest that they may be cotranscribed. |
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
LpxA and LpxB |
GO:0009245 |
lipid A biosynthetic process |
IEP: Inferred from Expression Pattern |
P |
Expression of genes on a DNA fragment derived from pLC25-43 from the Clarke-Carbon collection (PMID:???) was found to result in the accumulation of Lipid A disaccharide synthase (LpxB) and UDP-N-acetylglucosamine acyltransferase (LpxA) activity (Table 2). |
complete | ||
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