DH10B: Difference between revisions
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New page: == Strain == DH10B ---- Genotype F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ... |
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== Strain == | == Strain == | ||
DH10B | DH10B | ||
---- | ---- | ||
Genotype | |||
== Genotype == | |||
F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup> | F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup> | ||
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---- | ---- | ||
Comments | |||
== Comments == | |||
host for pUC and other α-complementation vectors; pBR322 | host for pUC and other α-complementation vectors; pBR322 | ||
useful for generating genomic libraries containing methylated cytosine or adenine residues | useful for generating genomic libraries containing methylated cytosine or adenine residues | ||
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---- | ---- | ||
References | |||
== References == | |||
1. Lorow, D. & Jessee, J. (1990) ''Focus''. 12,19 | 1. Lorow, D. & Jessee, J. (1990) ''Focus''. 12,19 | ||
Revision as of 16:16, 17 September 2008
Strain
DH10B
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
Mentioned from GIBCOTM website
Comments
host for pUC and other α-complementation vectors; pBR322 useful for generating genomic libraries containing methylated cytosine or adenine residues useful for plasmid rescue procedures
Copyed from GIBCO
References
1. Lorow, D. & Jessee, J. (1990) Focus. 12,19