PMID:8643639: Difference between revisions
Jump to navigation
Jump to search
Edited by wikibot |
Edited by wikibot |
||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
| Line 30: | Line 29: | ||
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | !Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
|-class='sortbottom' | |-class='sortbottom' | ||
|[{{SERVER}} | |[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Q46b53d690153f&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || || | ||
|} | |} | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.Q46b53d690153f--> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.Q46b53d690153f--> | ||
Revision as of 12:09, 30 April 2008
Tomer, G, Cohen-Fix, O, O'Donnell, M, Goodman, M and Livneh, Z (1996) Reconstitution of repair-gap UV mutagenesis with purified proteins from Escherichia coli: a role for DNA polymerases III and II.Proc. Natl. Acad. Sci. U.S.A. 93:1376-80
| Abstract | Using a cell-free system for UV mutagenesis, we have previously demonstrated the existence of a mutagenic pathway associated with nucleotide-excision repair gaps. Here, we report that this pathway can be reconstituted by using six purified proteins: UvrA, UvrB, UvrC, DNA helicase II, DNA polymerase III core, and DNA ligase. This establishes the minimal requirements for repair-gap UV mutagenesis. DNA polymerase II could replace DNA polymerase III, although less effectively, whereas DNA polymerase I, the major repair polymerase, could not. DNA sequence analysis of mutations generated in the in vitro reaction revealed a spectrum typical of mutations targeted to UV lesions. These observations suggest that repair-gap UV mutagenesis is performed by DNA polymerase III, and to a lesser extent by DNA polymerase II, by filling-in of a rare class of excision gaps that contain UV lesions. |
| Links | PubMed |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.