PMID:2125278: Difference between revisions
Jump to navigation
Jump to search
Edited by wikibot |
Edited by wikibot |
||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
| Line 20: | Line 19: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.W46a62eca3341b--> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.W46a62eca3341b--> | ||
[{{SERVER}} | [{{SERVER}}{{SCRIPTPATH}}/index.php/Special:TableEdit?id=63ce1649aab8b3180235b0af7618f667.0.W46a62eca3341b&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations] | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.W46a62eca3341b--> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.W46a62eca3341b--> | ||
Revision as of 11:54, 30 April 2008
Wigley, DB, Derrick, JP and Shaw, WV (1990) The serine acetyltransferase from Escherichia coli. Over-expression, purification and preliminary crystallographic analysis.FEBS Lett. 277:267-71
| Abstract | An expression vector has been constructed which increases the expression of serine acetyltransferase (SAT) from E. coli to 17% of the soluble cell protein. A novel purification procedure, using dye-affinity chromatography, allows purification of SAT to homogeneity. The enzyme has been crystallised from polyethylene glycol, in the presence of L-cysteine (an inhibitor of SAT). The crystals which diffract to beyond 3.0 A resolution are of the tetragonal spacegroup P4(1)2(1)2 (or P4(3)2(1)2) with cell dimensions a = b = 123 A, c = 79 A. Since ultracentrifugation and gel-filtration experiment indicate that purified SAT is a tetramer, there appears to be one-half tetramer in the asymmetric unit (Vm = 2.55 A3/Da). |
| Links | PubMed |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.