PMID:16738554: Difference between revisions
Jump to navigation
Jump to search
Edited by wikibot |
|||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
| Line 11: | Line 11: | ||
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] | |{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] | ||
|} | |} | ||
==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| Line 19: | Line 19: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.B46ab8696aabcb--> | |||
[{{SERVER}}/colipedia/index.php/Special:TableEdit?id=63ce1649aab8b3180235b0af7618f667.0.B46ab8696aabcb&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations] | [{{SERVER}}/colipedia/index.php/Special:TableEdit?id=63ce1649aab8b3180235b0af7618f667.0.B46ab8696aabcb&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations] | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.B46ab8696aabcb-- | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.B46ab8696aabcb--> | ||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Revision as of 22:03, 25 April 2008
Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.Mol. Syst. Biol. 2:2006.0008
| Abstract | We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/). |
| Links | PubMed |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.