galk2: Difference between revisions
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'''Notice:''' no direct refrence to the isolation of galactose negative mutants of galactokinase 2. Direct publication not identified for galactokinase 2. This shows the isolation of galactokinase mutants, but it should have been found in similar manner. Galk2 mutation is a newer discovery and not quite clear yet. <ref name=PMID: | '''Notice:''' no direct refrence to the isolation of galactose negative mutants of galactokinase 2. Direct publication not identified for galactokinase 2. This shows the isolation of galactokinase mutants, but it should have been found in similar manner. Galk2 mutation is a newer discovery and not quite clear yet. <ref name=PMID:11711531/>, <ref name=PMID:15750622/>, <ref name=PMID:14534577/> | ||
===References=== | ===References=== | ||
Revision as of 06:25, 21 February 2008
Notes
Mutagenesis: E coli bacteria was treated with UV radiation to induce mutation in galactose pathway [1]
Phenotype: Galactose is broken down through α-galactose-1-phosphate(GAL-1-P), uridine diphosphogalactose (UDPGal), and uridine diphosphoglucose (UDPG). Three enzymes are used to convert galactose to glycolytic intermediate. Under incubation, galactose mutants were given galactose and ATP and researchers measured the production of galactose-1-phosphate to determine galactokinase activity. Galactose negative mutants showed no galactokinase activity and therefore no production of galacose-1-phosphate. [2]
Notice: no direct refrence to the isolation of galactose negative mutants of galactokinase 2. Direct publication not identified for galactokinase 2. This shows the isolation of galactokinase mutants, but it should have been found in similar manner. Galk2 mutation is a newer discovery and not quite clear yet. [3], [4], [5]
References
- ↑ Morse, ML et al. (1956) Transduction in Escherichia Coli K-12. Genetics 41 142-56 PubMed
- ↑ KURAHASHI, K (1957) Enzyme formation in galactose-negative mutants of Escherichia coli. Science 125 114-6 PubMed
- ↑ Bai, S & Cao, X (2002) A nuclear antagonistic mechanism of inhibitory Smads in transforming growth factor-beta signaling. J Biol Chem 277 4176-82 PubMed
- ↑ Cui, Q et al. (2005) Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP. Oncogene 24 3864-74 PubMed
- ↑ Derynck, R & Zhang, YE (2003) Smad-dependent and Smad-independent pathways in TGF-beta family signalling. Nature 425 577-84 PubMed