pET-28b(+): Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older editNewer edit →
Pei (talk | contribs)
student
583 edits
edited by Pei via TableEdit
Pei (talk | contribs)
student
583 edits
No edit summary
Line 191: Line 191:




[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Kanamycin Marker Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:T7 Promoter]][[Category:MCS]][[Category:His Tag]][[Category:lacI]]
__NOTOC__
__NOTOC__

Revision as of 02:32, 4 February 2008

At a Glance

pET-28a-c(+), image provided by Novagen
Type

Cloning, Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
MCS
His Tag
lacI

Accession Number
edit table



Notes

The pET-28b(+) vector carries an N-terminal His•Tag®/thrombin/T7•Tag® configuration in addition to an optional C-terminal His•Tag sequence. The sequence is numbered by the pBR322 convention, resulting in the T7 expression region being reversed on the vector map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is included. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40]

  1. ↑ Antignani, A & Youle, RJ (2007) The cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), can deliver Bcl-XL as an extracellular fusion protein to protect cells from apoptosis and retain differentiation induction. J Biol Chem 282 11246-54 PubMed
  2. ↑ Baker, CS et al. (2007) CsrA inhibits translation initiation of Escherichia coli hfq by binding to a single site overlapping the Shine-Dalgarno sequence. J. Bacteriol. 189 5472-81 PubMed
  3. ↑ Braglia, P et al. (2007) Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae. Mol. Cell. Biol. 27 1545-57 PubMed
  4. ↑ Curti, E et al. (2007) Characterization of the helicase activity and substrate specificity of Mycobacterium tuberculosis UvrD. J. Bacteriol. 189 1542-55 PubMed
  5. ↑ Fischbach, MA et al. (2007) Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Proc. Natl. Acad. Sci. U.S.A. 104 11951-6 PubMed
  6. ↑ Frenkiel-Krispin, D et al. (2007) Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-VirE2 complex assembly by VirE1. J. Biol. Chem. 282 3458-64 PubMed
  7. ↑ Guan, C et al. (2007) Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota. Appl Environ Microbiol 73 3669-76 PubMed
  8. ↑ Tocchini-Valentini, GD et al. (2007) The dawn of dominance by the mature domain in tRNA splicing. Proc Natl Acad Sci U S A 104 12300-5 PubMed
  9. ↑ Wu, AT et al. (2007) PAWP, a sperm-specific WW domain-binding protein, promotes meiotic resumption and pronuclear development during fertilization. J. Biol. Chem. 282 12164-75 PubMed
  10. ↑ Azmi, I et al. (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. J. Cell Biol. 172 705-17 PubMed
  11. ↑ Schelert, J et al. (2006) Regulation of mercury resistance in the crenarchaeote Sulfolobus solfataricus. J Bacteriol 188 7141-50 PubMed
  12. ↑ Alt, JR et al. (2005) Mdm2 binds to Nbs1 at sites of DNA damage and regulates double strand break repair. J Biol Chem 280 18771-81 PubMed
  13. ↑ Breazeale, SD et al. (2005) A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose. J. Biol. Chem. 280 14154-67 PubMed
  14. ↑ Fennell-Fezzie, R et al. (2005) The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins. EMBO J 24 1921-30 PubMed
  15. ↑ Fischbach, MA et al. (2005) In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase. Proc. Natl. Acad. Sci. U.S.A. 102 571-6 PubMed
  16. ↑ Jilaveanu, LB et al. (2005) Dimeric SecA is essential for protein translocation. Proc. Natl. Acad. Sci. U.S.A. 102 7511-6 PubMed
  17. ↑ Klaus, SM et al. (2005) A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants. J. Biol. Chem. 280 5274-80 PubMed
  18. ↑ Klein, FA et al. (2005) Biochemical and NMR mapping of the interface between CREB-binding protein and ligand binding domains of nuclear receptor: beyond the LXXLL motif. J Biol Chem 280 5682-92 PubMed
  19. ↑ Korneeva, NL et al. (2005) Interaction between the NH2-terminal domain of eIF4A and the central domain of eIF4G modulates RNA-stimulated ATPase activity. J Biol Chem 280 1872-81 PubMed
  20. ↑ Kothe, M et al. (2005) Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase. Protein Sci 14 37-44 PubMed
  21. ↑ Li, Z et al. (2005) VHL protein-interacting deubiquitinating enzyme 2 deubiquitinates and stabilizes HIF-1alpha. EMBO Rep 6 373-8 PubMed
  22. ↑ Matuszewska, M et al. (2005) The small heat shock protein IbpA of Escherichia coli cooperates with IbpB in stabilization of thermally aggregated proteins in a disaggregation competent state. J Biol Chem 280 12292-8 PubMed
  23. ↑ Nagaike, T et al. (2005) Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. J. Biol. Chem. 280 19721-7 PubMed
  24. ↑ Okada, K & Hase, T (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. J. Biol. Chem. 280 20672-9 PubMed
  25. ↑ Reynolds, CM et al. (2005) A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant. J. Biol. Chem. 280 21202-11 PubMed
  26. ↑ Vaillancourt, FH et al. (2005) SyrB2 in syringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate- and O2-dependent halogenase. Proc Natl Acad Sci U S A 102 10111-6 PubMed
  27. ↑ Bae, NS et al. (2004) Human histone deacetylase SIRT2 interacts with the homeobox transcription factor HOXA10. J Biochem 135 695-700 PubMed
  28. ↑ Dong, TC & Cutting, SM (2004) The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis. J Biol Chem 279 43468-78 PubMed
  29. ↑ Ferreira, V et al. (2004) The classical activation pathway of the human complement system is specifically inhibited by calreticulin from Trypanosoma cruzi. J Immunol 172 3042-50 PubMed
  30. ↑ Im, YJ et al. (2004) The active site of a lon protease from Methanococcus jannaschii distinctly differs from the canonical catalytic Dyad of Lon proteases. J Biol Chem 279 53451-7 PubMed
  31. ↑ Ronning, DR et al. (2004) The carboxy-terminal portion of TnsC activates the Tn7 transposase through a specific interaction with TnsA. EMBO J 23 2972-81 PubMed
  32. ↑ Wenig, K et al. (2004) Structure of the streptococcal endopeptidase IdeS, a cysteine proteinase with strict specificity for IgG. Proc Natl Acad Sci U S A 101 17371-6 PubMed
  33. ↑ Zhang, YX et al. (2004) Characterization of a novel toxin-antitoxin module, VapBC, encoded by Leptospira interrogans chromosome. Cell Res 14 208-16 PubMed
  34. ↑ Ramelot, TA et al. (2003) Solution structure of Vibrio cholerae protein VC0424: a variation of the ferredoxin-like fold. Protein Sci 12 1556-61 PubMed
  35. ↑ Thoden, JB & Holden, HM (2003) Molecular structure of galactokinase. J Biol Chem 278 33305-11 PubMed
  36. ↑ Suárez, T et al. (2002) Biochemical defects in retina-specific human ATP binding cassette transporter nucleotide binding domain 1 mutants associated with macular degeneration. J. Biol. Chem. 277 21759-67 PubMed
  37. ↑ Kadkhodayan, S et al. (2000) Cloning, expression, and one-step purification of the minimal essential domain of the light chain of botulinum neurotoxin type A. Protein Expr. Purif. 19 125-30 PubMed
  38. ↑ Lecker, SH et al. (1999) Ubiquitin conjugation by the N-end rule pathway and mRNAs for its components increase in muscles of diabetic rats. J. Clin. Invest. 104 1411-20 PubMed
  39. ↑ Wang, D & Parrish, CR (1999) A heterogeneous nuclear ribonucleoprotein A/B-related protein binds to single-stranded DNA near the 5' end or within the genome of feline parvovirus and can modify virus replication. J. Virol. 73 7761-8 PubMed
  40. ↑ Chaussivert, N et al. (1995) Complex interactions between yeast TFIIIB and TFIIIC. J. Biol. Chem. 270 15353-8 PubMed

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 
     241 ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg 
     301 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 
     361 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 
     421 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 
     481 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 
     541 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 
     601 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 
     661 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 
     721 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 
     781 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 
     841 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 
     901 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 
     961 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 
    1021 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 
    1081 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 
    1141 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 
    1201 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 
    1261 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 
    1321 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 
    1381 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 
    1441 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 
    1501 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 
    1561 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 
    1621 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 
    1681 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 
    1741 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
    1801 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
    1861 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 
    1921 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 
    1981 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 
    2041 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 
    2101 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 
    2161 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 
    2221 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 
    2281 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 
    2341 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 
    2401 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 
    2461 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 
    2521 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 
    2581 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 
    2641 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 
    2701 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 
    2761 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 
    2821 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 
    2881 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 
    2941 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 
    3001 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 
    3061 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 
    3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
    3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
    3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
    3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
    3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
    3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
    3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
    3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
    3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
    3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
    3721 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
    3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
    3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
    3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg 
    3961 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 
    4021 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 
    4081 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 
    4141 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 
    4201 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 
    4261 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 
    4321 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 
    4381 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 
    4441 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 
    4501 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 
    4561 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 
    4621 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 
    4681 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 
    4741 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 
    4801 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca 
    4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat 
    4921 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 
    4981 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 
    5041 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 
    5101 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 
    5161 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 
    5221 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 
    5281 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 
    5341 gccgctacag ggcgcgtccc attcgcca
Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=pET-28b(%2B)&oldid=337644"